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The Gardens are located at 1 Old South Road, Bowral, on the north east corner of Kangaloon Road and Old South Road.
Entry into the fenced portion of the garden is through Gates 1 or 3 from the Old South Road carpark.
The unfenced eastern area of the gardens has no parking close by. Access is through the Old South Road carpark or the Alice Avenue northern entry.

GPS co-ordinates: -34.499072,  150.430447

Southern Highlands Botanic Gardens, 1 Old South Road Bowral NSW 2576.
Gardens are hatched red in the interactive map below.